Stimulation of Tryptophanase Synthesis in Escherichia coli by Cyclic 3’, S-Adenosine Monophosphate
نویسنده
چکیده
Cyclic 3’,5’-AMP stimulates the rate of tryptophanase synthesis in Escherichia coli treated with tris(hydroxymethyl)aminomethane and ethylenediaminetetraacetic acid and induced with tryptophan. A maximal effect occurs at 4 X low4 M cyclic 3’,5’-AMP and a half-maximal response at 1.5 X lo-4 M. The action of cyclic 3’,5’-AMP is specific for tryptophanase since the over-all rate of protein and RNA synthesis and the synthesis of the biosynthetic enzyme tryptophan synthetase are unaffected. One site of cyclic 3’,5’-AMP action appears to be after the transcription of DNA into messenger RNA since increased enzyme synthesis is observed in induced cells in which cyclic 3’,5’-AMP is added after messenger RNA synthesis is arrested by actinomycin D, proflavine, or inducer removal. The halflife of the messenger RNA for tryptophanase is 3 min and is unaffected by cyclic 3’,5’-AMP. Cyclic 3’,5’-AMP also fails to stimulate the conversion of tryptophanase precursor into active enzyme. Thus cyclic 3’,5’-AMP appears to act at the level of the polysome to increase the rate of polypeptide synthesis.
منابع مشابه
Short Papers Site of Action of 3',5'-cyclic Adenosine Monophosphate in Production of Tryptophanase in Escherichia Colz
3',5' cydic-AMP (CAMP) will stimulate the rate of tryptophanase synthesis in Esckrichia coli cultures induced with tryptophan. Adding CAMP after the initiation of messenger RNA synthesis was blocked by rifampicin, did not stimulate tryptophanase synthesis. This indicates that cAMP acts at initiation of either transcription or translation and not at the level of chain elongation of either the me...
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Cyclic 3’,5’-AMP stimulates the rate of tryptophanase synthesis in Escherichia coli treated with tris(hydroxymethyl)aminomethane and ethylenediaminetetraacetic acid and induced with tryptophan. A maximal effect occurs at 4 X low4 M cyclic 3’,5’-AMP and a half-maximal response at 1.5 X lo-4 M. The action of cyclic 3’,5’-AMP is specific for tryptophanase since the over-all rate of protein and RNA...
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